Structural deconstruction and enzymatic saccharification enhancement by acid catalyzed 1-n-butyl-3-methylimidazolium chloride pretreatment
Depolymerization of cellulose to glucose is partly inefficient due to the deleterious impact of cellulose crystallinity on enzymatic saccharification.In this work,we demonstrate how the enzymatic hydrolysis profile of cellulose in Arundo donax Linn.was influenced by crystal transformation.Cellulose I in A.donax was converted into cellulose II by using solid acid catalyzed ionic liquid(IL)1-n-butyl-3-methylimidazolium chloride(C4mimCl)pretreatment.The pretreatment also released abundance of reducing sugars.Cellulose II hydrate form was presented in the wet pretreated samples,whereas irreversibly converted into cellulose II after air-dried for 48 h.These crystal transformations enhanced saccharification rates significantly,accompanying by initial rates(defined as sugar released within the first 3 h)by up to 6.5-9.6 folds at an enzyme loading of 12 FPU/g substrate.The highest initial rate(75 g/kg/h)of glucose released was achieved for the cellulose II hydrate samples.However,similar cellulose conversion was observed after enzymatic hydrolysis for 48 h.Moreover,the yield of TRS and distribution in different liquors were dependent on the anti-solvents used.These discoveries provide new insights into a more economic pretreatment for bioethanol production.
Tingting You Liming Zhang Feng Xu
Beijing Key Laboratory of Lignocellulosic Chemistry,Beijing Forestry University,Beijing 100083,China
国际会议
第五届制浆造纸新技术国际研讨会(ISETPP)暨第三届国际造纸与环境学术大会(IPEC)
广州
英文
1-5
2016-11-07（万方平台首次上网日期，不代表论文的发表时间）