Optimization of preparation of NDV F Gene encapsulated in N-2-HACC-CMC nanoparticles

摘要：

　　In this study,the biodegradable materials N-2-hydroxypropyl trimethyl ammonium chloride chitosan(N-2-HACC)and N,O-carboxymethyl chitosan(CMC)are used as delivery carrier for the pVAX Ⅰ-F(o)-C3d6.The optimal preparation condition is as follows: concentration of N-2-HACC is 1.0 mg/ml,concentration of CMC is 0.85 mg/ml,concentration of pVAXⅠ-F(o)-C3d6 is 100 μg ml.The results show that the prepared N-2-HACCCMC/pFDNA NPs have regular round shape,smooth surface and good dispersion,the particle size is 310 nm,Zeta potential is 50 mV,the entrapment efficiency is 92%,the loading capacity is 51%(n=3).

作者: S S Li Y Zhang K Zhao X H Wang

作者单位: Key Laboratory of Microbiology,School of Life Science,Heilongjiang University,Harbin 150080,China Key Laboratory of Microbiology,School of Life Science,Heilongjiang University,Harbin 150080,China;Sc Heilongjiang University Library,Harbin 150080,China;School of Biological Science and Technology,Univ

会议类型: 国际会议

会议名称: 第二届新型材料与化学工业国际学术会议

会议地点: 三亚

会议语种:英文

页码: 1-5

在线出版日期: 2017-11-18（万方平台首次上网日期，不代表论文的发表时间）