Preparation and Characterization of the Magnetic Layered Double Hydroxide-DNA Delivery System
The purpose of this paper is to prepare and evaluate a gene delivery system resulted from the intercalation of DNA with magnetic layered double hydroxide (MLDH).The structure and property of the MLDH/DNA hybrids were studied using XRD, FTIR and TG characterization.The cytotoxicity and protection effect of MLDH were evaluated by MTT assay and gel electrophoresis assay.MLDH exhibited lower cytotoxicity than other inorganic nanoparticles.Under physiological conditions, MLDH could protect DNA from enzymatic degradation.Our results confirmed that MLDH can be used safely and effectively as non-viral vectors for gene targeting therapy in form of MLDH/DNA composite.
Magnetic layered double hydroxide Gene delivery system Nanoparticles Cytotoxicity Protection against degradation
GuoJing Gou QinYu Pei HuiQing Yao Yue Sun JianHong Yang Rui Wang
Department of Pharmaceutics, College of Pharmacy, Ningxia Medical University,Yinchuan, China 750004
国际会议
银川
英文
3-10
2017-08-16（万方平台首次上网日期，不代表论文的发表时间）