CRISPR/Cas9 mediated Stearoyl-CoA desaturase 1 knockout in primary goat mammary epithelial cell

摘要：

Introduction The CRISPR/Cas9 system has been shown to be a powerful genome-editing tool in a variety of species, including model organisms as well as crops and animals which are crucial to agriculture(Wang et al., 2015a, Wang et al., 2015b, Guo et al., 2016).Dairy goat is one of the most widely raised livestock in the world, and goat milk is the most important production of it.The monounsaturated fatty acids (MUFA)in the goat milk have been considered as beneficial fatty acids to human body health(Schwingshackl L,2014).MUFA are either obtained from diet or produced from delta-9 desaturation of saturated fatty acids,which catalyzed by stearoyl-CoA desaturase 1 (SCD1) (Sampath et al., 2007, Malodobra-Mazur et al.,2014).This process is also the rate-limiting step in MUFA synthesis.The objective of the present study was to determine the role of SCD1 in the milk fatty acids synthesis in goat mammary epithelial cells using the CRISPR/Cas9 mediated gene knockout technology.

作者: H.B.Tian J.Luo Z.F.Zhang

作者单位: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology,Northwest A & F University, Yangling, 712100, PR China

会议类型: 国际会议

会议名称: The 5h International Symposium on Dairy Cow Nutrition and Milk Quality(第五届“奶牛营养与牛奶质量国际研讨会)

会议地点: 北京

会议语种:英文

页码: 364-366

在线出版日期: 2017-05-05（万方平台首次上网日期，不代表论文的发表时间）