Paraoxonase 2 modulates a proapoptotic function in LS174T cells in response to quorum sensing molecule N-(3-oxododecanoyl)-L-homoserine lactone
Introduction The gut epithelium is coated with a thick mucus layer that functions as the first-line defensive barrier against invading microbes and pathogenic antigens 1,2.The mucus layer is composed of mucins (MUCs),digestive enzymes, antimicrobial peptides, and immunoglobulins 3,4.QS is an intercellular signaling mechanism that is thought to allow bacteria to coordinate behaviors at the population level 5.Many gram-negative bacteria use N-(3-oxododecanoyl)-homoserine lactone (C 12-HSL), a small lipid-soluble and membrane-permeant molecule as an autoinducer of QS 6.As the major epithelial barrier on the gut surface,MUCs secreted by goblet cells play an essential role on the maintenance of epithelial homeostasis.However, the effects of AHLs on goblet cells function remains unclear.We report for the first time that C12-HSL but not C4-HSL markedly decreases cell viability and induces apoptosis in a dose-and time-dependent manner in the goblet cell line LS 174T.
Liuping Cai Shivu Tao Yanwen Luo Bin He Jie Liu Yingdong Ni
Key Laboratory of Animal Physiology & Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu, China
国际会议
The 5h International Symposium on Dairy Cow Nutrition and Milk Quality(第五届“奶牛营养与牛奶质量国际研讨会)
北京
英文
369-372
2017-05-05(万方平台首次上网日期,不代表论文的发表时间)