Immune stimulant effects of a nucleotide-rich bakers yeast extract in the kuruma shrimp, Marsupenaeus japonicus
Immune stimulant effects of a nucleotide-rich bakers yeast extract (Vertex IG20) were investigated in the kuruma shrimp, Marsupenaeus japonicus by examining expression of anti-microbial peptides/proteins (AMPs) such as penaeidin (MjPen), crustin (MjCrus) and lysozyme (MjLyz) genes.Furthermore, to confirm that the bakers yeast extract-induced AMPs were functional, we also assessed the effect of its oral administration on resistance to Vibrio nigripulchritudo infection in the kuruma shrimp.Our results demonstrate that bakers yeast extract-injected and fed shrimps displayed a significant up-regulation of MjPen, MjCrus and MjLyz gene expression in the lymphoid organ.Moreover, significantly increased (P<0.01) resistance to the bacterial pathogen in term of better post infection survival (66.6%) was observed in the shrimp fed with the yeast extract-incorporated diet compared with the control diet fed group (8.3%).The present study indicates the immunostimulatory effects of the nucleotide-rich bakers yeast extract on the kuruma shrimp immune system and supports its potential use in aquaculture.
Kuruma shrimp mRNA transcript Penaeidin Crustin Nucleotides
G.Biswas H.Korenaga R.Nagamine T.Kono H.Shimokawa T.Itami M.Sakai
Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192,Japan Interdisciplinary Research Organization, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki TableMark Co., Ltd., Seasoning Business Group, 5-14, Haneda Asahicho Ota-ku, Tokyo 144-0042, Japan
国际会议
广州
英文
122-127
2017-03-01（万方平台首次上网日期，不代表论文的发表时间）