Separation and Purification of mulberry leaf γ-aminobutyric acid
In this study, mulberry leaf γ-aminobutyric acid (abbreviated GABA) was extracted from mulberry leaves using pulsed ultrasonic and microwave-assisted extraction method.The extracts were purified with column chromatography, and the purity was measured with the liquid chromatography.The results showed the best extraction conditions were : material-solvent ratio 1 (g) ∶34 (mL), microwave irradiation power at 144 W, and extraction time at 165 s.With this extraction condition, GABA yield was 0.583%.The crude GABA extracts were washed with ethanol to remove protein contaminates, and purified with S-8 macroporous resin and sephadex G-10, and 732-sulfonic acid cation exchange resin column chromatography.The final while GABA crystals had a purity of 90.3%.
mulberry leaves y-aminobutyric acid separation and purification
Chen Fangyan Chen Hengwen Zhong Yangsheng Lin Jianrong
College of Animal Science, South China Agricultural University, Guangzhou 510642, China
国际会议
The 9th China International Silk Conference (ISC2016)(第九届中国国际丝绸会议)
广西宜州
英文
413-422
2016-09-09（万方平台首次上网日期，不代表论文的发表时间）