Identification of Ancient Silk Using an Enzyme-linked Immunosorbent Assay

摘要：

　　In this study, an indirect ELISA was applied for specific identification of ancient silk.The process consists of preparing a tailored antibody (silk fibroin, SF), identifying the corresponding protein in ancient silks by enzyme-linked immunosorbent assay (ELISA).The anti-SF antibody exhibits high sensitivity and specificity for the identification of modern and ancient silks, and no cross-reactions with other possible interference antigens have been noted.Compared with other detecting methods, ELISA was sensitive, simple and specific.With such superior features, ELISA will become a frequently-used technique in the detection of proteins in ancient materials.

关键词： ancient silk identification ELISA

作者: Yi LIU Bing WANG Miaomiao LIU Zhiwen HU Hailing ZHENG Yang ZHOU

作者单位: Key Laboratory of Advanced Textile Materials and Manufacturing Technology, Ministry of Education,Zhe Institute of Textile Conservation, Zhejiang Sci-Tech University, Hangzhou 310018, China Key Scientific Research Base of Textile Conservation, State Administration for Cultural

会议类型: 国际会议

会议名称: The 9th China International Silk Conference (ISC2016)(第九届中国国际丝绸会议)

会议地点: 广西宜州

会议语种:英文

页码: 423-426

在线出版日期: 2016-09-09（万方平台首次上网日期，不代表论文的发表时间）