Identification of a Hemolin Protein from Actias selene Mediates Immune Response to Pathogens
Hemolins play a key role in development and innate immunity in insects.In this study, a hemolin cDNA sequence of 1412bp in Actias selene (As-HEM) were isolated and its open reading frames open reading frames (ORFs) were 420bp.The recombinant protein of As-HEM was expressed in Escherichia coli, and anti-As-HEM antibodies were prepared.Real-time quantitative PCR (RT-qPCR) and western blot results revealed that As-HEM mRNA and protein expressions were mostly detected in the hemocyte/hemolymph, while expressed at lower levels in midgut and fat body, and much lower amounts in other tissues tested.Also, the expressions of As-HEM mRNA and protein could be induced significantly after Beauveria bassiana, E.coli, Micrococcus luteus and nuclear polyhedrosis virus challenges.Agglutination assay revealed that recombinant As-HEM could promote the agglutination of E.coli in the presence of calcium.Our results suggested that As-HEM played an important role in the innate immunity of Actias selene.
Actias selene Hemolin Expression patterns Immune response
Cen Qian Fang Wang Baojian Zhu Lei Wang Guoqing Wei Yu Sun Sheng Li Chaoliang Liu
College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, PR China
国际会议
The 9th China International Silk Conference (ISC2016)(第九届中国国际丝绸会议)
广西宜州
英文
620-631
2016-09-09（万方平台首次上网日期，不代表论文的发表时间）