Analysis of Differential Proteome Related Sclerotium Disease at Early Stage of Mulberry Fruits
The fungus disease of sclerotinia is the mainly biotic stress factors which severely impact the mulberry fruits development, and do destructive harm on the productivity and quality of mulberry fruits.In this study, the molecular mechanism was discussed at pathogenic fungus infecting mulberry, by using the diseased and normal young fruits of variety Da 10 as the experimental material.The two-dimensional electrophoresis (2-DE), mass spectrometry and bioinformatics technique were used to isolate and identify the differential expression proteins.The results showed that 45 proteins were differentially expressed more than 1.5 times, in which 18 proteins were up-regulated while 27 proteins were down-regulated compared with normal young fruits through the 2-DE map images with IPG-IEF/SDS-PAGE analysis;In which, 34 proteins identified by MALDI-TOF/TOF MS were related to many physiological processes, mainly including material and energy metabolism, also involving in gene expression regulation,oxidation-reduction reaction, cellular component and plant stress responses.Subcellular localization of the identified proteins were analyzed by bioinformatics technique.The results showed that mulberry young fruits can regulate the expression levels of multiple proteins localized mainly in cytoplasm and the organelles to reply sclerotium disease infection.The novel findings about these pathogenesis-related proteins will provide valuable information to further study the pathogenesism of sclerotinia disease and disease-resistant molecular breeding in mulberry.
Mulberry Sclerotium disease Pathogenesis-related proteins Differential proteome Mass spectrometry analysis
Pei-feng YIN Chuan-le ZHAO Hua GUAN Li-fen WANG Jian-zhong TAN
Department of Horticulture, School of Architecture, Soochow University, Suzhou, 215123 China;School Department of Horticulture, School of Architecture, Soochow University, Suzhou, 215123 China
国际会议
The 9th China International Silk Conference (ISC2016)(第九届中国国际丝绸会议)
广西宜州
英文
632-637
2016-09-09（万方平台首次上网日期，不代表论文的发表时间）