Dietary Effects on Enzymatic Activity and Gene Expression of α-Amylase in Antheraea pernyi Midgut
The digestive tract of insects exhibits multiple, diverse and unique combination of enzymes to digest food material depending on dietary content of a range of host plants.Alpha-amylase is an important class of enzyme which is required for the digestion of starch to ready energy source required for the optimal growth and development of insects.In this study, we cloned and characterized amylase gene (Ap-Amy) from Antheraea pernyi (Lepidoptera: Saturniidae).The gene length consisted of 1794-bp open reading frame (ORF) encoding 597 amino acid residues.Expression analysis of Ap-Amy by RT-PCR indicated that the gene was detected in eggs and larvae stages and mostly transcribed in midgut of A.pernyi larvae, corresponding to major place of digestion.Amylase activity and gene expression were higher in tussah feeding on Quercus acutissima than Quercus liaotungensi.This research is helpful to screen high-feed-efficiency A.pernyi varieties and provide new strategies to increase the production of tussah industry.
Antheraea pernyi amylase gene expression natural diets
Yong Wang Yiren Jiang Shenglin Shi Ruisheng Yang Li Qin
College of Bioscience and Biotechnology, Shenyang Agricultural University;Liaoning Engineering and Technology Resource Center for Insect Resource, Shenyang, Liaoning 110866, China
国际会议
The 9th China International Silk Conference (ISC2016)(第九届中国国际丝绸会议)
广西宜州
英文
648-656
2016-09-09（万方平台首次上网日期，不代表论文的发表时间）