Micropropagation and bulblet transplantation of Lilium cernuum
Lilium cernuum is a national key resource of protected wild plants in China.To propagate and produce this wild lily commercially, we have conducted tissue culture and bulblet micropropagation, enlargement, and transplantation.We used bulb scales and shoots as explants to establish micropropagation systems.The germination rate of the inner scales was lower than that of the outer scales, which was again lower than that of the middle scales.The optimal medium for adventitious bud differentiation from bulb scales and axillary buds from shoot segments was MS + 6-BA 0.5 mg L-1 + NAA 0.2 mg L-1, and the optimal medium for proliferation of adventitious and axillary buds was MS + 6-BA 0.5 mg L-1+ NAA 0.1 mg L-1, with a proliferation coefficient up to 2.03 and 0.92, respectively.Tests showed that padobutrazol (PP333) is the major factor affecting the enlargement of bulblets.The optimum concentration of PP333 was 30 mg L-1.So far, we have obtained more than 1500 in vitro bulblets of six populations.We transplanted bulblets of circumference around 3.5 cm after 2 days hardening.Those potted in nutrient medium and grown in the solar greenhouse with daily management survived better than those planted in wild fields.The bulblets planted in Beijing sprouted continuously with high germination rate over 3 years, and blossomed in July 2015.This research provides an effective pathway for large-scale micropropagation of L.cernuum.
bulb scales wild lily shoot segment PP333 transplantation
H.Y.Xiao H.Hao Q.L.Liu
Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing, China
国际会议
The XII International Symposium on Flower Bulbs and Herbaceous Perennials(第12届国际球宿根花卉学术研讨会)
昆明
英文
17-24
2016-06-28（万方平台首次上网日期，不代表论文的发表时间）