Bulblet regeneration in vitro from bulb-scales of Fritillaria thunbergii
Fritillaria thunbergii (Liliaceae), commonly known in Chinese as zhebei, is a perennial plant native to China with important medicinal and ornamental value.Wild populations are at a risk of rapid eradication, and are now very difficult to find.In order to protect wild resources and to set up an efficient system of rapid propagation,sections of bulb-scales from the vegetative period were used to regenerate bulblets in vitro.Surface-disinfected sections were cultured in Murashige and Skoog (MS)medium containing 35 g L-1 sucrose and supplemented with different types,concentrations and combinations of plant growth regulators α-naphthaleneacetic acid (NAA), 6-benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KT).Bulblets were successfully induced from scale sections after 12 weeks of culture via two pathways.In the first pathway, callus was induced within 5 weeks from scale sections on solid MS medium that was supplemented with 0.5 mg L-1 BA and 2.0 mg L-1 NAA.After placing callus at low temperature (4℃) for 3 weeks, bulblets developed on the same medium within 4 weeks (11.3 bulblets explant-1).In the second (less effective) pathway, bulblets could be induced directly from scale sections on solid MS medium supplemented with 2.0 mg L-1 BA and 0.5 mg L-1 NAA, or 1.0 mg L-1 BA and 0.1 mg L-1 2,4-D, resulting in 8.2 and 7.8 bulblets explant-1, respectively.This is a simple but effective protocol for the mass propagation of F.thunbergii.
fritillaries traditional Chinese medicine tissue culture organ micropropagation conservation
L.H.Hao J.A.Teixeira da Silva X.N.Yu
College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China P.O.Box 7, Miki-cho Post Office, Ikenobe 3011-2, Kagawa-ken 761-0799, Japan College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China;Beijing Key La
国际会议
The XII International Symposium on Flower Bulbs and Herbaceous Perennials(第12届国际球宿根花卉学术研讨会)
昆明
英文
25-29
2016-06-28（万方平台首次上网日期，不代表论文的发表时间）