Colorimetric Sensor Strips Based on Enzyme Immobilized Nanofiber for Pesticides Detection
With the widespread use of pesticides, poisoning frequently occurs due to residues.Therefore, an efficient method of detection is essential.The design principle in this study is based on organophosphorus pesticides would inhibit acetylcholinesterase (AChE) catalyzed indole acetate (IA) hydrolysis.To improve the sensitivity, we use nanofibers as a substrate for enzyme immobilization.Moreover, material structure and surface chemical groups are also designed.The cellulose acetate nanofibers membranes are prepared via electrospinning, and an alkali treatment and pyromellitic anhydride esterification are carried out, eventually creating the substrate for AChE immobilization.Also, the effect of fixed temperature and pH of the enzyme recovery are measured to maximize the efficient loading amount and optimize the conditions for subsequent detection.Subsequently, effects of the enzyme concentrations, IA concentration and inhibition time are also investigated by detecting the color changes utilizing UV spectroscopy.The optimal temperature of the enzyme is 37℃, the best immobilization pH value is 7.5, the optimal IA concentration is 50 mM, the optimal enzyme concentration is 5 U/mL, and the best pesticide suppression time is 15 min.Sensor with portable, fast, and accurate properties, is expected to pesticide residues on fruits and vegetables in daily life can be detected.
electrospinning nanofiberslEnzyme immobilization acetylcholinesterase colorimetric sensors
De Li Mohammed Awad Abedalwafa Yan Li Lu Wang
Key Laboratory of Textile Science and Technology, Ministry of Education, Donghua University, China Key Laboratory of Textile Science and Technology, Ministry of Education, Donghua University, China;D
国际会议
2017 Sino-Africa International Symposium on Textiles and Apparel (SAISTA2017)(2017中非纺织服装国际论坛)
上海
英文
16-21
2017-11-18（万方平台首次上网日期，不代表论文的发表时间）