Rhizosphere Effect of Tall Fescue on the Structure and Functions of the Bacterial Community in Response to Polycyclic Aromatic Hydrocarbons in an Aged Agricultural Soil

摘要：

　　To investigate the bacterial mechanism of PAH degradation in an aged-contaminated agricultural soil planted with tall fescue (Festuca arundinacea), a rhizo-box experiment was conducted for 60 days.The residual PAH concentrations in soils were quantified.Shifts in bacterial community structure were assessed based on cDNA, and the abundance and activity of total bacteria and PAH-degrader were monitored by quantification of 16S rDNA, PAH-ring hydroxylating dioxygenase (PAH-RHDα) genes and their transcripts.We found soil bacteria were dominated by Alphaproteobacteria (45.2%) and Gammaproteobacteria (25.8%).Tall rescue positively affected the abundance and activity of total bacteria in the soil., and stimulated RHDα gram-negative gene expression while inhibited RHDα gram-postive gene expression.These suggest that PAH dissipation in rhizosphere soil can be explained by modifications in the microbial community structure, an increase in the abundance of PAH-degraders, and RHDα GN gene expression enhancement during the incubation.

关键词： rhizosphere effect gene expression bacterial community RHDα gene phytoremediation

作者: GONG Zongqiang GUO Meixia James ROOKES David CAHILL Lingxue KONG

作者单位: Key Biotechnology Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applie Institute of Frontiers Materials, Deakin University, Geelong, Victoria 3200, Australia School of Life and Environmental Science, Deakin University, Geelong, Victoria 3200, Australia

会议类型: 国际会议

会议名称: The 2017 International Symposium on Environmental Science and Technology (2017环境科学与技术国际会议)

会议地点: 北京

会议语种:英文

页码: 82-94

在线出版日期: 2017-09-19（万方平台首次上网日期，不代表论文的发表时间）