Research on Two Different Functional Types of N-methyltransferase Genes in Camellia sinensis
N-methyltransferases (NMTs) are the key enzymes in regulating the biosynthesis of purine alkaloids in Camellia sinensis, which catalyze three methylation reactions from xanthosine to caffeine sequentially, including xanthosine→7-methylxanthosine,7-methylxanthine→theobromine and theobromine→caffeine.Currently, two functional NMT genes TCS1 and PCS1 had been cloned from tea plants.TCS1, which catalyzed the last two reactions in caffeine biosynthesis, came from caffeine-containing tea plants, while PCS1, which catalyzed the second step in caffeine biosynthesis, came from caffeine-free tea plants.The function and source of NMT genes implied the complexity of caffeine biosynthesis modulated by NMTs in Camellia sinensis.Here two new NMT genes with different catalytic functions were isolated from the same caffeine-containing tea plants in this study, and the catalytic functions of these genes were compared.
REN Qiu-jing YAN Chang-yu CHEN Xiao-fang XU Yu-hua JIAO Yi CHEN Zhong-zheng LI Bin
College of Food Science, South China Agricultural University, Guangzhou, 510642, China
国际会议
International Tea Symposium 2014(ITS 2014)(国际茶叶学术研讨会)
杭州
英文
230-232
2014-09-10（万方平台首次上网日期，不代表论文的发表时间）