Comparative Proteomic and Metabolic Analysis between Bud and Young Expanding Leaves of Tea Plant (C.sinensis L.)
Tea (Camellia sinensis L.) is a perennial woody plant widely cultivated for the production of a popular non-alcoholic beverage, which has received much attention due to its attractive aroma, pleasant and numerous medicinal benefits.To understand the processes of leaf growth, development and secondary metabolite production, an isobaric tag for relative and absolute quantitation (iTRAQ) analysis was used to separate differentially expressed proteins between bud and young expanding leaves of tea plant (C.sinensis L.).A total of 235 differentially expressed proteins were identified.Among them,116 proteins were up-regulated and 119 proteins were down-regulated in the young expanding leaves when compared with tea bud.According to the biological functional properties, these proteins were classified into seven functional categories: metabolism,nucleic acid metabolism, protein metabolism, biological regulation and signal transduction, stress/defense/detoxification, transport and unknown function.This study provides a basis for further functional characterization of differentially expressed leaf proteins, which can help us understand the molecular basis of secondary metabolism in tea and will be a source of candidate genes for tea cultivar innovation in future.
Camellia sinensis L. iTRAQ leaf development secondary metabolite
Qin Li Juan Li Shuoqian Liu Haiyan Lin Zhonghua Liu Jianan Huang
Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha,Huna Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha,Huna National Research Center of Engineering Technology for Utilization of Functional Ingredients from Bo
国际会议
International Tea Symposium 2014(ITS 2014)(国际茶叶学术研讨会)
杭州
英文
323-325
2014-09-10（万方平台首次上网日期，不代表论文的发表时间）