Highly sensitive electrogenerated chemiluminescence biosensor for galactosyltransferase activity and inhibition detection using gold nanorod and enzymatic dual signal amplification
As one of the glycosyltransferase involved in protein glycosylation,β-1,4-galactosyltransferase (Gal T) plays an important role in the cellular process and progression of cancer.Here,using the bovine serum albumin conjugated N-acetylglucosamine (GlcNAc-BSA) as a receptor to fabricate bioelectrode interface,a sensitive electrochemiluminescence (ECL) biosensor was constructed for Gal T activity analysis based on the recognition between artocarpus integrifolia lectin (AIA) and galactose,integrating with a dual signal amplification strategy from the xanthine oxidase (XOD) and AIA multi-labeled gold nanorod nanoprobes.The gold nanorods promoted the electron transfer on the electrode interface and were also employed as carriers of AIA and XOD due to their large surface area.Furthermore,both the gold nanorods and XOD catalyzed the ECL reaction,which dramatically amplified the ECL signal of luminol in the presence of hypoxanthine (HA) and oxygen.The as-proposed ECL biosensor exhibited high sensitivity on the detection of Gal T activity and a detection limit of 9 × 10-4U mL-1 was obtained.This assay was successfully applied for the analysis of Gal T activity expression in different cell lines and inhibition detection,showing great potential for glycosyltransferase activity analysis and inhibitors screening in clinic diagnostics.
Glycobiology Galactosyltransferase Electrogenerated chemiluminescence Biosensor Gold nanorod Dual signal amplification
Zhuhai Chen Ling Zhang Yang Liu Jinghong Li
Department of Chemistry,Beijing Key Laboratory for Analytical Methods and Instrumentation,Tsinghua University,Beijing 100084,China
国际会议
南京
英文
83-89
2016-12-23（万方平台首次上网日期，不代表论文的发表时间）