A label-free impedimetric cytosensor based on galactosylated gold-nanoisland biointerfaces for the detection of liver cancer cells in whole blood
Circulating tumor cells (CTCs),as potential cancer biomarkers,play a crucial role in cancer diagnosis,prognosis and treatment.However,there are still significant challenges due to their rarity.In this paper,a label-free and ultrasensitive electrochemical impedance cytosensor was developed by a galactosylated gold-nanoisland (Gal- GNI) biointerface for the capture and detection of rare cancer cells (HepG-2) in whole blood samples.By mimic the natural multivalent biorecognition systems,the biointerface was designed to capture the target cancer cells by the specific binding between galactosyl ligands of Gal-GNI and over-expressed asialoglycoprotein receptors (ASGP-R) on the surface of HepG-2.From the results of electrochemical impedance spectroscopy (EIS) and fluorescence spectroscopy,the nanostructured Gal-GNI provides an improved electron-transfer rate,specific detection for HepG-2,and good biocompatibility for viable captured cells.The capture of HepG-2 to the EIS cytosensor increased the electron-transfer resistance,with a good correlation with the logarithm of the concentration from 1.0 × 102 to 1.0 × 105 cells mL-1,with a low detection limit of 30 cells mL-1.This cytosensing strategy has good reproducibility with the RSD of 1.9% in blood samples,indicating that it can be used as a potential noninvasive assay tool for the early diagnosis and therapy of tumor.
CTC detection Nanostructured biointerfaces EIS cytosensor Carbohydrate-lectin interaction Label-free
Juan Liu Jieni Cai Hui Chen Song Zhang Jilie Kong
Department of Chemistry,Fudan University,Shanghai 200433,PR China
国际会议
南京
英文
103-108
2016-12-23（万方平台首次上网日期，不代表论文的发表时间）