Mediator enhanced glucose detection using organic-inorganic hybrid supramolecular assembly on gold electrodes
In this study,a new organic-inorganic material,based on an alpha-metatungstate H2W12O406- cluster,was synthesized via the hydrothermal method by using aminopyridinium (APy)+ cations as the hybrid material,and characterized by FT-IR and UV spectra analysis.The hybrid composite was immobilized on a gold electrode decoratedwith gold nanoparticles (AuNPs) and themodified electrode exhibited excellent electrocatalytic activities towards the reduction of H2O2 at neutral pH with a response time of b10 s.The H2O2 response at the modified electrode displays a linear response ranging from 40 μM to 10 mM.Based on the H2O2 reduction activity,the (APy)6H2W12O40 composite was successfully used for the fabrication of a novel enzymatic glucose biosensor.The glucose oxidase enzyme (GOx) was immobilized onto the hybrid composite on AuNPs/gold electrodes; the (APy)6H2W12O40 composites were used as bothmediation and bridgingmolecules.A multilayer architecture was constructed from hybrid POM alternating with (GOx),using glutaraldehyde as a covalent attachment cross-linker.Cyclic voltammetry (CV),SWV and chronoamperometry were used to determine the electrochemical properties of the mediator and characterize its reaction with (GOx).The resulting glucose biosensor exhibits awide linear response for glucose from 40 μM to 10 mM with a sensitivity of 15.0 μA mM-1.
Hybrid polyoxometalates Hydrogen peroxide detection Glucose oxidase Enzyme biosensor Electron transfer mediator
Yosra Sahraoui Sana Chaalia Abderrazak Maaref Amor Haddad Francois Bessueille Nicole Jaffrezic-Renault
Laboratory of Interfaces and Advanced Materials,Faculty of Sciences of Monastir,Avenue of Environmen Laboratory of Materials and Crystallochemistry,Superior Institute of Applied Sciences and Technology Laboratory of Interfaces and Advanced Materials,Faculty of Sciences of Monastir,Avenue of Environmen University of Lyon,Institute of Analytical Sciences,UMR CNRS-UCBL-ENS 5280,5 rue de la Doua,69100 Vi
国际会议
南京
英文
190-197
2016-12-23（万方平台首次上网日期，不代表论文的发表时间）