Rolling circle amplification in electrochemical biosensor with biomedical applications
Signal amplification is vital in biosensor fabrication,especially for the sensitive analysis of target molecules.Rolling circle amplification (RCA) is an isothermal nucleic acid amplification technique.In a typical RCA process,a short DNA or RNA primer is extended under the catalysis of a polymerase to form a long single stranded DNA or RNA using a circular DNA template.In recent years,various approaches have been proposed to improve the RCA technique,so this nucleic acid amplification technique has become an attractive tool for biosensor fabrication,especially for the development of electrochemical biosensors with biomedical applications.Owing to the accumulation of RCA products on electrode surface,the electrochemical signal can be greatly amplified after an elaborated design so as to improve the detection sensitivity.Therefore,a variety of biomolecular targets such as genes,microRNAs,proteins,with ultra-low abundance have been successfully detected using the developed RCA-based electrochemical biosensors.In this review,we will summarize the improvement of the RCA technique and its application for the development of electrochemical biosensors with biomedical applications.We will also comment on the recent progress of RCA-based electrochemical biosensors,making an outlook on the trends of the related research fields in the future.
Electroanalysis Biosensor Rolling circle amplification Signal amplification
Chang Feng Xiaoxia Mao Yucai Yang Xiaoli Zhu Yongmei Yin Genxi Li
State Key Laboratory of Pharmaceutical Biotechnology and Collaborative Innovation Center of Chemistr Center for Molecular Recognition and Biosensing,School of Life Sciences,Shanghai University,Shanghai Department of Oncology,The First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,PR State Key Laboratory of Pharmaceutical Biotechnology and Collaborative Innovation Center of Chemistr
国际会议
南京
英文
223-232
2016-12-23（万方平台首次上网日期，不代表论文的发表时间）