An ultrasensitive electrochemiluminescence immunosensor based on zeolitic imidazolate frameworks encapsulating spherical graphite crystals
Here,an ultrasensitive electrochemiluminescence (ECL) immunosensor for the detection of cardiac troponin Ⅰ (cTnⅠ) was developed by using zeolitic imidazolate frameworks encapsulating spherical graphite crystals (CQDs@ZIF-8) as luminophore.Firstly,luminescent spherical graphite crystals (CQDs) were encapsulated in the zeolitic imidazolate framework (ZIF-8) nanocrystals.To improve the poor conductivity of CQDs@ZIF-8,Au nanoparticles were synthesized on the surface of CQDs@ZIF-8 to form AuNPs/CQDs@ZIF-8.Furthermore,L-cysteine (L-cys) as the coreactant of CQDs was immobilized on the AuNPs to enhance the ECL signal of CQDs.Then,the obtained L-cys/AuNPs/CQDs@ZIF-8 was introduced to construct an ultrasensitive ECL immunosensor for the detection of cTnⅠ.As a result,the proposed immunosensor displayed excellent analytical performances for the detection of cTnⅠ with a linear range from 1 fg μL -1 to 10 pg μL-1 and a detection limit of 0.33 fg μL-1.
Electrochemiluminescence Immunosensor Cardiac troponin Ⅰ Spherical graphite crystals Zeolitic imidazolate frameworks
Ruhua Zang Ying He Ruo Yuan Yaqin Chai
Key Laboratory of Luminescent and Real-Time Analytical Chemistry,Ministry of Education,College of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,Peoples Republic of China
国际会议
南京
英文
284-288
2016-12-23（万方平台首次上网日期，不代表论文的发表时间）