An ultrasensitive sandwich-type electrochemical immunosensor for carcino embryonie antigen based on supermolecular labeling strategy
A sandwich-type electrochemical immunosensor for detection of carcino embryonie antigen (CEA) was fabricated based on supermolecular labeling strategy.In this work,β-cyclodextrin (β-CD)-based host-guest recognition was used to immobilize capture antibody and label detection antibody.β-CDs functionalized multiwalled carbon nanotube (CDs-MWCNT) was used to immobilize adamantanecarboxylic acid (ADA) functionalized capture antibody onto electrode surface based on host-guest interaction between ADA and β-CD.The detection antibody was first labeled with ferrocenecarboxylic acid (Fc) and then attached to magnetic Fe3O4@SiO2 core-shell nanoparticles based on the inclusion interaction between Fc and β-CD.Fc acts as not only a linker molecule but also electroactive specie.The multiplex labeling of Fc on Fe3O4@SiO2 nanoparticles enabled the signals amplification for CEA after the specific immunoreaction,which was monitored by square wave voltammetry.Under the optimal conditions,the immunosensor displayed a relatively wide linear range from 0.001 ng mL-1 to 15 ng mL-1 with a detection limit of 0.33 pg mL-1 for the detection of CEA.The newly designed immunosensor showed a promising potential application in clinical diagnosis.
Magnetic nanoparticles Multiwalled carbon nanotube Carcino embryonie antigen Host-guest recognition
Zhaoqing Yan Hongmin Ma Dawei Fan Lihua Hu Xuehui Pang Jian Gao Qin Wei Qi Wang
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong,School of Chemistry and Ch School of Material Science and Engineering,University of Jinan,Jinan 250022,PR China
国际会议
南京
英文
289-295
2016-12-23（万方平台首次上网日期，不代表论文的发表时间）