One-pot electrodeposition of a composite film of glucose oxidase, imidazolium alkoxysilane and chitosan on a reduced graphene oxide-Pt nanoparticle/Au electrode for biosensing
Acomposite film of glucose oxidase (GOx),imidazolium alkoxysilane (ImAS,a silane-ionic liquid (IL) bifunctional reagent) and chitosan (CS) was electrodeposited in a one-pot manner on a reduced graphene oxide (RGO)-Pt nanoparticle (PtNP) modified Au electrode for biosensing of glucose.Cyclic voltammetry electroreduction of coexisting p-benzoquinonewas used to lift the electrode-surface pH for the GOx-ImAS-CS deposition,as a result of deprotonation of CS and hydrolyzation/condensation of ImAS at lifted pH.The RGO-PtNP composite was prepared through mild room-temperature chemical reduction of graphene oxide and H2PtCl6·6H2O by formic acid,as characterized by UV-visible/FT-IR/Raman spectrophotometry,scanning electron microscopy and electrochemical techniques.The GOx-ImAS-CS/RGO-PtNP/Au electrode was used for glucose biosensing by anodically detecting the enzymatically generated H2O2 at 0.55 V versus SCE.Under the optimal conditions,the GOx-ImAS- CS/RGO-PtNP/Au electrode showed a linear amperometric response to glucose concentration from 2.0 μM to 5.5 mM with a sensitivity of 84.4 μA mM-1 cm-2 and a limit of detection of 0.02 μM(S/N=3),which is superior to Gox-CS/RGO-PtNP/Au,Gox-(IL-NH2)-CS/RGO-PtNP/Au,Gox-(3-aminopropyltriethoxysilane)-CS/RGO- PtNP/Au and Gox-ImAS-CS/Au electrodes as well as many reported enzyme electrodes fabricated using similar materials.
Glucose oxidase-imidazolium alkoxysilane-chitosan composite film pH-induced electrodeposition of enzyme film Graphene oxide-Pt nanoparticle composite Au electrode Amperometric glucose biosensing
Feng Wu Ting Huang Yangjian Hu Xin Yang Qingji Xie
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research(Ministry of Education), Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research(Ministry of Education), Chemistry and Chemical Engineering Department,Huaihua College,Huaihua 418008,China
国际会议
南京
英文
296-303
2016-12-23（万方平台首次上网日期，不代表论文的发表时间）