Portable detection of clenbuterol using a smartphone-based electrochemical biosensor with electric field-driven acceleration
We have developed a fast,highly sensitive and low-cost biosensing system for the detection of clenbuterol (CLB),using a homemade mobile electrochemical device with an electric field-driven acceleration strategy.This system consists of an embedded circuit in smartphone for signal processing and a screen-printed carbon electrode (SPCE) modified with multi-walled carbon nanotubes (MWNTs) and goat anti mouse-immunoglobulin G (IgG) sensing layer (MWNTs-I-layer).CLB monoclonal antibody was assembled through its binding to the surface-confined antibody.Such modified electrodes were used for rapid and sensitive amperometric immunosensing detection of CLB.Horseradish peroxidase-coupled CLB (CLB-HRP) competed with free CLB in the samples to bind the monoclonal antibody.By using this mobile system,we could detect CLB ranging from 0.3 ng·mL-1 to 100 ng·mL-1 with the detection limit of 0.076 ng·mL-1.The whole competitive-type detection process was finished within 6 min.We expect this device can meet the requirements for field detection of various food security-related species.
Electrochemistry Portable Sensor Smartphone Electrical field
Yanzhi Dou Zhineng Jiang Wangping Deng Jing Su Shixing Chen Haiyun Song Ali Aldalbahi Xiaolei Zuo Shiping Song Jiye Shi Chunhai Fan
Key Laboratory for Organic Electronics & Information Displays(KLOEID),Institute of Advanced Material Division of Physical Biology & Bioimaging Center,Shanghai Synchrotron Radiation Facility,Shanghai In Key Laboratory of Food Safety Research,Institute for Nutritional Sciences,Shanghai Institutes for Bi Chemistry Department,King Saud University,Riyadh 11451,Saudi Arabia Kellogg College,University of Oxford,Oxford OX2 6PN,UK
国际会议
南京
英文
339-344
2016-12-23（万方平台首次上网日期，不代表论文的发表时间）