Confining nanohybrid of CdTe quantum dots and cytochrome P450 2D6 in macroporous ordered siliceous foam for drug metabolism
In this work,a simple and accurate in vitro drug metabolism strategy was proposed by using macroporous ordered siliceous foam (MOSF) as a nanoreactor for confining the nanohybrid of CdTe quantum dots (QDs) and cytochrome P450 2D6 (CYP2D6).After CYP2D6 was coupled to QDs through the carbodiimide coupling chemistry,the resulting nanohybrid of CYP2D6/QDs exhibited fluorescence emission at 645 nm,which could be used as a photocatalyst for drugmetabolism.The energy level of CYP2D6 was located between the conduction band (CB) and valence band (VB) of QDs,which provided the possibility of the electron transferring from the CB of QDs to CYP2D6.Tramadol was chosen as the model substrate.Under the irradiation of a white-light,the photocurrent increased with the addition of tramadol in a wide linear range from 4.0 μM to 100 μM.The apparent Michaelis-Menten constant was measured to be 3.6 μM.The high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) confirmed the production of metabolite of o-desmethyltramadol.Such nanoreactor provided a suitable environment to confine a mass of enzyme,as well as to maintain their catalytic activities for highly effective drug metabolic reactions.This showed promising potential for immobilizing various enzymes and other biomolecules in biomimetic metabolism study.
Macroporous ordered siliceous foam Nanoreactor Quantum dots Cytochrome P450 2D6 Drug metabolism
Ling Jiang Ying Li Wei Wei Anran Liu Yuanjian Zhang Songqin Liu
Key Laboratory of Environmental Medicine Engineering,Ministry of Education,Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device,School of Chemistry and Chemical Engineering,Southeast University,Nanjing 210096,PR China
国际会议
南京
英文
345-350
2016-12-23（万方平台首次上网日期，不代表论文的发表时间）