Highly sensitive electrochemiluminescent DNA biosensor based on hydrazide-modified graphene quantum dots and hemin/ G-quadruplex DNAzyme
Graphene quantum dots (GQDs),an alternative of conventional luminescent reagents,has been widely used in electrochemiluminescent (ECL) analysis because of its low cost,non-toxicity and ease of preparation.To further improve the ECL signal of GQDs,we herein succeeded in synthesizing hydrazide modified graphene quantum dots (HM-SGQDs).In comparison with GQDs,HM-SGQDs possessed abundant luminol like units,thus greatly enhancing the ECL intensity.On the other hand,luminol involved ECL system typically needed the participation of H2O2 as a coreactant,while hemin/G-quadruplex DNAzyme (hGQ) DNAzyme could efficiently catalyze the decomposition of H2O2.Accordingly,taking p53 gene as a model,a novel ECL DNA biosensor was developed based on HM-SGQDs and hGQ DNAzyme.The experimental results indicated target DNA can be quantified in a linear range from 100 fM to 100 nM with a detection limit of 66 fM (S/N = 3).Meanwhile,the discrimination of single-base mismatch was also achieved with the proposed analytical approach,suggesting broad potential applications of nucleic acid-related clinical diagnosis.
Electrochemiluminescent biosensor Hydrazide modified graphene quantum dots Hemin/G-quadruplex DNAzyme p53 gene
Xiaoying Wang Li Liu Zhaoyin Wang Zhihui Dai
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials,School of Chemistry and Materials Science,Nanjing Normal University,Nanjing 210023,PR China
国际会议
南京
英文
351-355
2016-12-23（万方平台首次上网日期，不代表论文的发表时间）