Photoelectrochemical biosensor for acetylcholinesterase activity study based on metal oxide semiconductor nanocomposites
Acetylcholinesterase (AChE) is a well-known serine hydrolase and its dysfunction could disturb cholinergic neurotransmission which is related to the pathogenesis of neurodegenerative disorders.In this report,a photoelectrochemical biosensor based on metal oxide semiconductor nanocomposite was fabricated to investigate the effect of cadmium ion (Cd2+) on the activity of AChE.The photoelectrochemical nanocomposite was prepared by anodic oxidation of titanium(Ti) foil to formtitaniumdioxide (TiO2) nanotubes (TNs) array followed by cathodic deposition of zinc oxide nanorods (ZnONRs) onto the TNs.AChE was immobilized on the obtained nanocomposites and the biosensor showed enhanced photoelectrochemical response under visible light irradiation.The experimental results showed that Cd2+ exhibited interesting dose-dependent and time-dependent effects on AChE activity.Specifically,high concentration of Cd2+ inhibited while low level of Cd2+ could stimulate the activity of AChE.These findings are of great significance for the study of enzyme activity influenced by metal ions and related pathogenesis investigation of neurodegenerative disorders.
Photoelectrochemical biosensor TiO2 nanotubes Nanocomposite Acetylcholinesterase Cadmium ion
Qilin Huang Ya Wang Li Lei Zhiai Xu Wen Zhang
School of Chemistry and Molecular Engineering,East China Normal University,Shanghai 200241,PR China; School of Chemistry and Molecular Engineering,East China Normal University,Shanghai 200241,PR China
国际会议
南京
英文
377-382
2016-12-23（万方平台首次上网日期，不代表论文的发表时间）