Novel graphene/Au-CdS:Eu composite-based electrochemiluminescence immunosensor for cancer biomarker detection by coupling resonance energy transfer and enzyme catalytic reaction
Recent studies show that ECL resonance energy transfer (ECL-RET) coupled with H2O2-related enzymatic reactions is an efficient strategy for designing excellent ECL biosensors with high sensitivity.Here we find that a ternary composite named graphene/Au-CdS:Eu QDs demonstrated ECL-RET effect with Au nanorods (Au NRs) and horseradish peroxidase (HRP) on the Au NRs could catalyze hydrogen peroxide reduction in the presence of hydroquinone (HQ).Therefore,we developed an efficient dual-quenching ECL immunosensor based on the novel ternary composite with high ECL intensity coupled with H2O2-related enzymatic reactions and ECL-RET strategy from Au NRs.This ultrasensitive ECL sensing strategy combined the advantages of the excellent ECL sensing properties of graphene/Au-CdS:Eu QDs,and dual-quenching effect and achieve sensitive detection of alpha fetoprotein (AFP).
Graphene/Au-CdS:Eu ternary composite Resonance energy transfer Au NRs Horseradish peroxidase ECL immunosensor
Meirong Cui Ruizhen Yu Xiaomeng Wang Hong Zhou Jing Liu Shusheng Zhang
Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Sha Linyi Cancer Hospital,Linyi 276005,PR China Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers,College of Chemistry an
国际会议
南京
英文
410-417
2016-12-23（万方平台首次上网日期，不代表论文的发表时间）