Cardiovascular toxicity Evaluation of Silica Nanoparticles in Endothelial Cells and Zebrafish Model
Environmental exposure to nanomaterials is inevitable as nanomaterials become part of our daily life, and as a result, nanotoxicity research is gaining attention.However, most investigators focus on the evaluation of overall toxicity instead of a certain organism system.In this regard, the evaluation of cardiovascular effects of silica nanoparticles was preformed in vitro and in vivo.Its worth noting that silica nanoparticles induced cytotoxicity as well as oxidative stress and apoptosis.ROS and apoptosis were considered as major factor to endothelial cells dysfunction, involved in several molecular mechanisms of cardiovascular diseases.In vivo study,mortality, malformation, heart rate and whole-embryo cellular death were measured in zebrafish embryos.Results showed that silica nanoparticles induced pericardia toxicity and caused bradycardia.We also examined the expression of cardiovascular-related proteins in embryos by western blot analysis.Silica nanoparticles inhibited the expression of p-VEGFR2 and p-ERK1/2 as well as the downregulation of MEF2C and NKX2.5, revealed that silica nanoparticles could inhibit the angiogenesis and disturb the heart formation and development.In summary, our results suggested that exposure to silica nanoparticles is a possible risk factor to cardiovascular system.
Silica nanoparticles Cardiovascular effects Endothelium Zebrafish Nanotoxicity
Junchao Duan Yongbo Yu Yang Li Yang Yu Zhiwei Sun
School of Public Health, Capital Medical University, Beijing, 100069, P.R.China School of Public Health, Jilin University, Changchun, Jilin, 130021, P.R.China School of Public Health, Capital Medical University, Beijing, 100069, P.R.China;School of Public Hea
国际会议
北京
英文
43-64
2013-06-01（万方平台首次上网日期，不代表论文的发表时间）