Aims: To investigate the association of tag-SNPs and haplotype structures of the CIDEA gene with obesity in a Han Chinese Population.Methods: Five single nucleotide polymorphisms (SNPs) (rs1154588/V115F, rs4796955/SNP1, rs8092502/SNP2, rs12962340/SNP3 and rs7230480/SNP4) in the CIDEA gene were genotyped in a case-control study.Genotyping was performed using the Sequenon Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry iPLEX platform.Results: There were significant differences between the obese and control groups in genotype distributions of V115F (P<0.001), SNP1 (P =0.006) and SNP2 (P=0.005).Carriers of V115F-TT, SNP1-GG and SNP2-CC genotypes had a 2.84-fold (95% CI:1.73-4.66), 2.19-fold (95% CI: 1.09-4.38) and 4.37-fold (95% CI: 1.2 1-1 5.08) increased risk for obesity, respectively.Haplotype analysis showed that GTTC (SNP1/SNP2/V115F/SNP4) had 1.41-fold (95% CI: 1.02-1.95) increased risk for obesity;whereas, haplotype TTGC had 0.48-fold (95% CI: 0.24-0.96) decreased risk for obesity.Using the multifactor dimensionality reduction (MDR) method, the best model including SNP1, SNP2, V115F and SNP4 polymorphisms was identified with a maximum testing accuracy to 59.32% and a perfect cross-validation consistency of 10/10 (P=0.01 l).Logistic analysis indicated that there was a significant interaction between SNP1 and Vll5F associated with obesity.Subjects having both genotypes of SNP1/GG and V115F/TT were more susceptible to obesity in the Han Chinese population (OR=2.66, 95%:1.22-5.80).Conclusions: Genotypes of V115F/TT, SNP1/GG and SNP2/CC and haplotype GTTC of CIDEA gene were identified as risk factors for obesity in the Han Chinese population.The interaction between SNP1 and V 1 15F could play a joint role in the development of obesity.
Chinese Association study Obesity CIDEA Polymorphism Haplotype
Jingjing Wu Ling Zhang Jie Zhang Ying Dai Lili Bian Manshu Song Alyce Russell Wei Wang
Department of Epidemiology and Biostatistics, School of Public Health,Capital Medical University, Be Beijing Municipal Key Laboratory of Clinical Epidemiology, Beijing, 100069, China Faculty of Medicine, the University of Hong Kong, Hong Kong, China Yuetan Community Health Service Center Affiliated to Fuxing Hospital, Beijing, 100045, China College of Life Science, Graduate University of Chinese Academy of Sciences, Beijing, 100049, China Systems and Intervention Research Centre for Health, School of Medical Sciences, Edith Cowan Univers