Soy Isoflavone Attenuates Brain Mitochondrial Oxidative Stress Induced by Beta-Amyloid Peptides 1-42 Injection in Lateral Cerebral Ventricle
The aim of this study is to investigate whether soy isoflavone (SIF) reduces oxidative stress and improves the antioxidant ability in mitochondria of rat brain damaged by injection of beta-amyloid peptides 1-42 (Aβ1-42).Forty Wistar rats were randomly divided into control, Aβ1-42, SIF + Aβ1-42 and SIF groups according to body weight.The rats in SIF + Aβ1-42 group and SIF group were intragastrically administrated SIF suspension in 0.5 % CMC-Na for 28 days, whereas the rats in control group and Aβ1-42 group were administrated the same volume of 0.5 % CMC-Na.On day14, the rats in Aβ1-42 group and SIF + Aβ1-42 group were injected with Aβ1-42 into lateral cerebral ventricle with physiological saline.The rat brains were then sampled, and brain mitochondria were isolated.After this, the mitochondrial membrane potential (MMP) and mitochondrial redox state were measured.The contents of brain nuclear factor E2-related factor (Nrf2) and heme oxygenase-1 (HO-1) protein in brain tissue were quantitated by Western blot.The results showed that SIF maintained the MMP, elevated the reduced glutathione / oxidized glutathione (GSH/GSSG) ratio, and increased glutathione peroxidase (GPx) and manganese superoxide dismutase (MnSOD) protein expression in brain mitochondria.Additionally, SIF reversed Aβ1-42-induced down-regulation of the protein expression of Nrf2 and HO-1 in brain tissue.These results indicated that SIF could alleviate the oxidative damage and maintain the redox imbalance in brain mitochondria damaged by Aβ1-42.These might be resulted from regulation of the Nrf2/HO-1 pathway.
soy isoflavone beta-amyloid peptides oxidative stress mitochondria brain
Juan Ding Huanling Yu Weiwei Ma Yuandi Xi Xia Zhao Linhong Yuan Jinfang Feng Rong Xiao
国际会议
北京
英文
140-153
2013-06-01（万方平台首次上网日期，不代表论文的发表时间）