Wheat proteogenomics: Mapping proteins and grain phenotype to the genome
The unique transcripts from a range of wheat gene transcripts datasets and coding sequences predicted from the wheat genome survey sequences (International Wheat Genome Sequencing Consortium; IWGSC)were resources used to establish a dataset of predicted protein entities with their respective molecular weights estimated.This dataset was then cross-referenced to available data for the molecular weights of proteins reported in the literature as well as from our own analyses of grain protein in samples from structured mapping populations.The chromosomal locations from a mapping population of 225 doubled haploid lines using high throughput MALDI-TOF analyses could be compared to predicted locations of proteins determined from the chromosome arm-based analysis of the wheat genome survey sequences.A pilot study on the proteogenomic analysis of wheat utilized published tandem mass spectrometry spectra from proteins separated on 2D gels, and this study validated annotations defined by AUGUSTUS gene predictions (using the maize model).We found 170 peptides in total, mapped to 76 known genes and 5novel genes.A more complete analysis using gene models predicted from FL-cDNAEST and RNA-seq evidence and mass spectra from shotgun mass spectrometry will provide a higher proteome coverage and ultimately provide a more comprehensive insight into the gene space within the wheat genome.The study highlighted some technical challenges in resolving proteins close to each other in molecular weight.This can now be addressed with newer mass spectroscopy technologies.The available dataset of predicted proteins was also analyzed for epitopes of potential interest for identifying grain proteins that may cause health issues in consumers of wheat-based products.
B.Chapman P.Moolhuijzen W.Ma M.Bellgard Y.Yan S.Wang A.Juhasz F.Bekes R.Appels
Center for Comparative Genomics, Murdoch University, Perth, WA 6150, Australia Capital Normal University,Beijing, China Agricultural Institute, Center for Agricultural Research of the Hungarian Academy of Sciences,Marton FBFD Pty Ltd, Beecroft, NSW 2119, Australia
国际会议
The 11th International Gluten Workshop(第十一届国际谷蛋白学术研讨会)
北京
英文
2-6
2012-08-12（万方平台首次上网日期，不代表论文的发表时间）