Monitoring of storage proteins: Accumulation and impact of high temperature in wheat kernels using proteomics
Proteomics analysis of gluten proteins was carried out on manually dissected starchy endosperm of three bread wheat cultivars: Récital, Arche and Tamaro.The albumins and globulins of the developing endosperm were first extracted and 21 stages of development were analyzed for the cultivar Récital.Proteomics analysis of storage proteins was also carried out for eight stages of development from the late period of endosperm cell division to physiological maturity.Based on quantitative variations in these storage proteins, principal component analysis revealed four major phases during their accumulation.Hierarchical cluster analysis revealed 10 major profiles, providing evidence for differences in the kinetics of protein accumulation.Identification of individual gliadins and glutenins showed that the protein accumulation profiles differed for these two groups.The storage protein profiles were compared to recently identified profiles of albumin-globulin proteins.A similar analysis was performed on cultivars Arche and Tamaro (medium and very high quality, respectively) which were subjected to two temperature regimes during the post-anthesis period: 28/15℃ (high temperature treatment) versus 23/11℃ (control) day/night air temperature.Although significant differences were observed in the gliadin:glutenin ratio between the high temperature treatment and the controls during the grain filling period, the ratios at maturity showed no significant differences between stressed and control for each cultivar.Interestingly, the very high quality cultivar Tamaro had far fewer fluctuations in its gliadin:glutenin ratio than cv.Arche, indicating differences in the genetic regulation of gluten protein synthesis among cultivars.
A.Tasleem-Tahir E.Bancel P.Martre G.Branlard
INRA, UMR 1095 GDEC, 5 Chemin de Beaulieu, 63039 Clermont-Ferrand, France;Blaise Pascal University,UMR1095 GDEC, F-631 77 Aubière, France
国际会议
The 11th International Gluten Workshop(第十一届国际谷蛋白学术研讨会)
北京
英文
13-17
2012-08-12（万方平台首次上网日期，不代表论文的发表时间）