Proteomic analysis of mature kernels of Fusarium graminearum-infected transgenic bread wheat expressing PGIP
Fusarium head blight (FHB), a worldwide wheat disease, has a detrimental effect both on productivity and qualitative properties of wheat flour.To evaluate the impact of FHB on the wheat flour we compared the proteomes of mature kemels obtained from Fusarium graminearum-infected spikes and control bread wheat plants.The same analysis was also performed on mature seeds of control and F.graminearum-infected transgenic bread wheat plants expressing a Poly-Galacturonase Inhibiting Protein (PGIP) that possesses partial resistance to FHB.DIGE investigation of metabolic proteins and A-PAGE and SDS-PAGE analysis of storage proteins (gliadins and glutenins) extracted from mature seeds did not identify any component differentially accumulated between the infected plants and noninfected controls, between infected and non-infected transgenic plants, or between infected and noninfected transgenic and control plants.Conversely, total starch content showed a significant difference between infected and non-infected control plants, with the infected control plants showing a reduced total starch content.Similarly, there was a significant difference between infected transgenic and control plants, with the transgenic plants showing higher total starch contents.These results indicate that moderate infection of FHB does not cause significant changes in the wheat seed proteome, but does reduce the total seed starch content.Moreover, the expression of PGIP protects the grain from starch decrease following F.graminearum infection.
R.DOvidio P.Laino M.Janni E.Botticella M.S.Di Carli E.Benvenuto K.Lilley D.Lafiandra S.Masci
Dipartimento di Scienze e Tecnologie per lAgricoltura, le Foreste, la Natura e lEnergia, Universit Dipartimento di Scienze e Tecnologie per lAgricoltura, le Foreste, la Natura e lEnergia, Universit Dipartimento di Scienze e Tecnologie per lAgricoltura, le Foreste, la Natura e lEnergia, Universit ENEA, Dipartimento BIOTEC, Sezione Genetica e Genomica Vegetale, C.R.Casaccia, Rome,Italy Department of Biochemistry, University of Cambridge, Cambridge, U.K
国际会议
The 11th International Gluten Workshop(第十一届国际谷蛋白学术研讨会)
北京
英文
18-21
2012-08-12（万方平台首次上网日期，不代表论文的发表时间）