The trafficking of wheat prolamins in rice endosperm
Transgenic rice was used to study the pattem and pathway of deposition of wheat high molecular weight (HMW) subunit 1Dx5 within rice endosperm using specific antibodies to determine whether it is deposited in the same or different protein bodies to rice storage proteins, and whether it is located in the same or separate phases within them.The protein distribution and the expression pattem of HMW subunit 1Dx5 in transgenic rice endosperm at different stages of development were also studied using tissue printing, light and electron microscopy.The use of high molecular weight glutenin subunits (HMW-GS) specific antibodies showed that subunit 1Dx5 was expressed mainly in the subaleurone cells of the endosperm and that it was deposited in both types of protein body present in the rice endosperm; derived from the ER and containing prolamins, and derived from the vacuole and containing glutelins.In addition, new types of protein bodies were also formed within the endosperm cells.Our results suggest that the HMW Dx5 protein could be trafficked by either the ER or vacuolar pathways, possibly depending on the stage of development, and that segregation of gluten proteins may occur both between and within protein bodies.
M.Oszvald L.Tamás P.R.Shewry P.Tosi
E(o)tv(o)s Loránd University, Department of Plant Physiology and Molecular Plant Biology, Budapest, Rothamsted Research, Harpenden, U.K
国际会议
The 11th International Gluten Workshop(第十一届国际谷蛋白学术研讨会)
北京
英文
38-42
2012-08-12（万方平台首次上网日期，不代表论文的发表时间）