A model for the transcriptional regulation of low molecular weight glutenin genes in wheat
Transcriptional regulation of LMW glutenin genes was investigated in-silico using publicly available sequence and expression data.Genes were grouped into different low molecular weight (LMW)glutenin types and their promoter profiles were determined using cis-acting regulatory element databases and published results.Based on promoter sequences and expression characteristics, LMW glutenin genes might be transcribed following two different mechanisms.Differences observed in their expression could be related to differences in the promoter sequences.The 24 variants of 12 storage protein-specific promoter motifs that were identified mainly belong to DOF, bZIP and Myb type transcription factor recognition sites.The various elements belong to conserved non-coding regulatory regions (CREs) and could act in different ways.There are regions in which regulatory elements, such as the GCN4 motifs found in the-300 element region, could serve as key factors in tissue-specific expression.Some other elements, like the further complete endosperm boxes in the-600 region or the individual prolamin box variants might modulate the level of expression.Based on the relatively small distance between different cis-acting promoter motifs, possible DOF-bZIP, DOF-Myb, bZIP-Myb interactions can be identified.
A.Juhász Sz.Makai E.Sebestyén L.Tamás E.Balázs
Applied Genomics Department, Agricultural Institute, Centre for Agricultural Research, HAS,Martonvás Department of Plant Physiology and Molecular Plant Biology, E(o)tv(o)s Loránd University, Budapest,
国际会议
The 11th International Gluten Workshop(第十一届国际谷蛋白学术研讨会)
北京
英文
47-51
2012-08-12（万方平台首次上网日期，不代表论文的发表时间）