Variation and classification of toxic epitopes related to celiac disease among α-gliadin genes from four Aegilops genomes
α-gliadins are associated with human celiac disease.A total of 23 non-interrupted full open α-gliadin gene reading frames and 19 pseudogenes were cloned and sequenced from the C, M, N and U genomes of 4 diploid Aegilops species.Sequence comparisons of α-gliadin genes from Aegilops and Triticum species demonstrated extensive allelic variations in the Gli-2 loci of the 4 Aegilops genomes.Specific structural differences included the compositions and variations of 2 polyglutamine domains(QI and QII) and 4 T cell stimulatory toxic epitopes.The average numbers of glutamine residues in the QI domain in the C and N genomes and the QII domain in the C, N and U genomes were much higher than those in Triticum genomes, and the QI domain in C and N genomes.The QII domain in the C, M,N and U genomes displayed greater length variations.Interestingly, the types and numbers of 4 T cell stimulatory toxic epitopes in α-gliadins from the 4 Aegilops genomes were significantly less than those in common wheat and its progenitors.Study of the relationships among the structural variations of the two polyglutamine domains and distributions of 4 T cell stimulatory toxic epitopes resulted in theα-gliadin genes from Aegilops and Triticum genomes being classified into three groups.
J.Li S.L.Wang S.S.Li P.Ge X.H.Li W.J.Ma F.J.Zeller S.L.K.Hsam Y.M.Yan
College of Life Science, Capital Normal University, Beijing 100048, China Centre for Comparative Genomics,Murdoch University and Western Australia Department of Agriculture ( Department of Plant Breeding, Technical University of Munich, D-85354 Freising-Weihenstephan, German
国际会议
The 11th International Gluten Workshop(第十一届国际谷蛋白学术研讨会)
北京
英文
52-61
2012-08-12（万方平台首次上网日期，不代表论文的发表时间）