Optimization and application of capillary electrophoresis for rapid separation and characterization of water-soluble proteins in wheat grains
Separation and analysis of water-soluble proteins (WSP) are important in understanding the fundamentals of the wheat grain proteome.However, due to their high degrees of heterogeneity and complexity in composition, separating WSP is generally difficult and relevant methodologies are not yet fully developed.Capillary electrophoresis (CE) is one of the analytical methods currently used for protein separation and characterization.In the present study, a CE method was optimized for rapid separation and characterization of water-soluble proteins (WSP) in wheat grains.The established method was tested in various applications, including wheat varieties and germplasm identification,as well as protein synthesis and accumulation during different grain development stages and under different environmental conditions.The characteristic CE pattems of a range of bread wheat cultivars and related species were readily identified.The synthesis and accumulation patterns of wheat WSP during grain development, as well as their stabilities in different environments, were also investigated.The technical advances presented in this paper appear to be useful for wheat cultivar and germplasm identification, as well as for genetic and breeding research.
C.Han Z.Yu S.Feng D.Lv X.Yan G.Chen X.Li W.Ma Y.Yan
College of Life Science, Capital Normal University, Beijing 100048, China Western Australian Department of Agriculture and Food and State Agriculture Biotechnology Center, Mu
国际会议
The 11th International Gluten Workshop(第十一届国际谷蛋白学术研讨会)
北京
英文
137-142
2012-08-12（万方平台首次上网日期，不代表论文的发表时间）