DNA sequence variations of four Wx alleles generating polymorphic Wx-A1 protein for decreased amylose content in wheat
Waxy (Wx) genes encode granule-bound starch synthase I (also called Wx protein) responsible for amylose synthesis of cereal grain starch.In this study, four wheat Wx-A1 alleles, viz.Wx-A1c,-A1e,-A1i and-A1j producing polymorphic Wx proteins were studied at the DNA sequence level.It had been suggested that apparent amylose contents conferred by the alleles increase in the order of WxA1e (=waxy phenotype) <-A1i<-A1c<-A1j (=amylose level of wild type Wx-A1a, or about 20%).DNA sequencing of these alleles detected SNPs and insertion/deletion variations, of which a particular SNP causing amino acid changes in Wx-A1e and-A1c was identified as the likely factor responsible for decreased amylose.On the other hand, a transposable-like element of 376 bp present in the 3UTR of Wx-A1i most probably lowered the levels of Wx protein and amylose.The fourth allele, Wx-A1j,possessed four SNPs, of which two altered amino acids of the Wx-A1j protein and should cause the protein polymorphism.Based on the DNA sequence determination, functional markers for Wx-A1c,-A1e and-A1i were developed.
M.Yamamori C.Guzman
NARO Institute of Crop Science, Tsukuba, 305-8518, Japan University of Córdoba, Córdoba,14071, Spain
国际会议
The 11th International Gluten Workshop(第十一届国际谷蛋白学术研讨会)
北京
英文
209-212
2012-08-12（万方平台首次上网日期，不代表论文的发表时间）