Responses of GSH and GST in Liver of Mossambica Tilapia under Four Typical Brominated Flame Retardants Stress in Vitro
(0) Brominated flame retardants (BFRs) are emerging persistent organic pollutants widely present in the enviorment This study reports in vitro effects of four kinds of BFRs including BDE-47,BDE-209,HBCD and TBBPA on oxidative stress (glutathione (GSH) content,and activities of glutathione S-transferase (GST) using a plate-reader method.Mossambica tilapia liver supematant were exposed in vitro to four kinds of BFRs for 1 h at different doses ranging from 0.1 ng/L to 1000 μg/L.For antioxidant enzymes acitivities and nonenzymatic antioxidant molecule,the results indicated that the same antioxidant parameter had the similar response trend to the 4 BFRs.As to hepatic GSH content,a biphasic response was observed,i.e.initial increased at the lower concentrations,and then decreased at the higher concentrations,while GSH content in the exposed groups were higher than that in the control at all mentioned concentrations.However,the GST activity was inhibited by the four types of BFRs.Our results suggested that the liver antioxidants adaption potentials may serve as a surrogate biomarkers to BFR exposure.
brominated flame retardants (BFRs) in vitro oxidative stress
WANG Haiyan HAN Daxiong HAN Jacai XIAO Dan
Third Institute of Oceanography, SOA, Xiamen 361005, China School of Pharmaceutical Sciences Xiamen University, Xiamen 361102, China Pingtan Entry-Exit Inspection &Quarantine Bureau of P.R.C, Fuzhou, China Third Institute of Oceanography, SOA, Xiamen 361005, China;School of Pharmaceutical Sciences Xiamen
国际会议
2015 International Symposium on Environmental Science and Technology (2015 ISEST)(2015环境科学与技术国际会议)
重庆
英文
131-134
2015-11-18（万方平台首次上网日期，不代表论文的发表时间）