3-D quantitative tracking of phagosomes using quantitative phase microscopy

摘要：

　　We present a method for quantitative tracking of phagosomes without labeling.Current imaging methods,used for phagosome research,have limitations in live-cell quantitative analysis.We reconstruct three-dimensional (3-D) refractive index (RI) tomograms from two-dimensional holograms with changing illumination angle.RI information enables to obtain intracellular phagosomes quantitatively.Time-lapse imaging provides 3-D informations of phagosom movement.Collectively,we propose that quantitative phase imaging techniques can be a useful tool for phagocytosis research.

作者: Jonghee Yoon Kyoohyun Kim Jaehwang Jung YongKeun Park

作者单位: Department of Physics,Korea Advanced Institute of Science and Technology,Daejeon 305-701,Republic of Korea

会议类型: 国际会议

会议名称: 2014 Asia Communications and Photonics Conference(ACP2014)(2014亚洲通信与光子学大会)

会议地点: 上海

会议语种:英文

页码: 1-2

在线出版日期: 2014-11-11（万方平台首次上网日期，不代表论文的发表时间）