Label-free quantitative imaging of lipid droplets using quantitative phase imaging techniques
We present a label-free imaging method for studying lipid droplets (LDs).LDs are lipid storage organelles in most eukaryotic cells.Recently,many studies have reported that LDs are related pathologic conditions including obesity,viral infection,and inflammatory diseases.Fluorescent proteins have been widely used to image LDs,but photobleaching and photodamage are limiting factors in live-cell imaging.Three-dimensional (3-D) refractive index (RI) tomograms were reconstructed from multiple holographic images with various illumination angles.We calculate mass and size of LDs from reconstruct 3-D RI tomograms.Time-lapse imaging provides 3-D dynamics of LDs.We propose that quantitative phase imaging techniques can be an invaluable tool for investigating pathophysiology of LDs.
Seo Eun Lee Kyoohyun Kim Jonghee Yoon Ji Han Heo HyunJoo Park Chulhee Choi YongKeun Park
Department of Biological sciences,Korea Advanced Institute of Science and Technology,Daejeon 305-701 Department of Physics,Korea Advanced Institute of Science and Technology,Daejeon 305-701,Republic of Department of Bio and Brain Engineering,Korea Advanced Institute of Science and Technology,Daejeon 3
国际会议
2014 Asia Communications and Photonics Conference(ACP2014)(2014亚洲通信与光子学大会)
上海
英文
1-2
2014-11-11（万方平台首次上网日期，不代表论文的发表时间）