Expression of Telomerase Reverse Transcriptase during the Malignant Transformation of Cadmium-Induced Cells

摘要：

　　The objective of the present study was to investigate human telomerase reverse transcriptase (hTERT) mRNA and protein expressions during the cadmium chloride-induced malignant transformation of human bronchial epithelial (16HBE) cells.Fluorescence quantitative PCR (FQ-PCR) and Western blot analyses were performed to detect the hTERT mRNA and protein expressions in normal 16HBE cells,cadmium chloride-transformed 16HBE cells,and tumorigenic cells from nude mice inoculated with cadmium chloride-transformed 16HBE cells.Under the inner standard of GAPDH,the hTERT mRNA expression was significantly higher at different stages of malignant transformation (cadmium chloride-transformed 16HBE cells at passages 15 and 35 and tumorigenic cells from nude mice) than in normal 16HBE cells,and increased with the development of malignancy (P<0.01).In addition,hTERT protein expression increased with the development of malignancy.These findings demonstrate that hTERT expression is related to cadmium chlorideinduced malignant transformation.Cadmium chloride-induced malignant transformation is involved in changes in the hTERT activity,and might be an early event in cadmium chloride-induced malignant transformation.

关键词： Cadmium Chloride Human Bronchial Epithelial Cells Malignant Transformation Telomerase Reverse Transcriptase

作者: Min Wang Yixiong Lei

作者单位: School of Public Health,Guangzhou Medical University,Guangzhou,China

会议类型: 国际会议

会议名称: The International Conference on Environmental Pollution and Public Health(2014年环境污染与大众健康学术会议)

会议地点: 上海

会议语种:英文

页码: 129-133

在线出版日期: 2014-04-12（万方平台首次上网日期，不代表论文的发表时间）