Identification of Differentially Expressed MicroRNAs from Warm Season and Cold Season Yak Subcutaneous Fat
MicroRNAs(miRNAs) are a family of-22 nucleotide small RNA molecules that regulate gene expression by fully or partially binding to their complementary sequences.Recently,many studies have shown the miRNAs played important roles in many biological processes in various species.However, to date no miRNAs have been reported to modulate subcutaneous development in yak.In order to better understand the role of miRNAs in subcutaneous fat metabolism of yak, two small RNAs libraries pooled from cold and warm season subcutaneous adipose tissues of three individuals were constructed respectively, and sequenced using an Illumina/Solexahighthroughput sequencer.A total of 23,626,630 clean reads were obtained from the cold season library and 23,423,315 clean reads from the warm season library.59 differentially expressed miRNAs were identified,44 miRNAs in warm season were significantly up-regulated with P ≤0.01,while 15 miRNAs were significantly down-regulated with P≤0.01.Bioinformatics of KEGG pathway analysis and GO term enrichment suggested that these differentially expressed miRNAs involved in different pathways and target genes may regulate differently fat deposition.Taken together, our study provides the first evidence for better understanding the differential expression and mechanisms of miRNA in yak subcutaneous fat deposition.
Chu Min La Huan Feng Yucheng Wu Xiaoyun Yan Ping
Key Laboratory for Yak Genetics, Breeding & Reproduction Engineering of Gansuprovince,Institute of L Datong Yak Breeding Farm of Qinghai Province, Datong, 810102, China
国际会议
The 5th International Conference on Yak(第五届国际耗牛大会)
兰州
英文
92-94
2014-08-27（万方平台首次上网日期，不代表论文的发表时间）