Effects of Chromatin Modifying Agent on Epigenetic of Yak Donor Cells and Cloned Embryos in vitro Development
Interspecies somatic cell nuclear transfer (iSCNT), a powerful tool in basic scientific research, has been widely used to increase and preserve the population of endangered species.However, abnormal epigenetic reprogramming of donor nucleus after SCNT is thought to be the main cause of low cloning efficiency.Previous studies have demonstrated that the treatment of intraspecies cloned embryos with epigenetic agent significantly improved nuclear-cytoplasmic reprogramming and in vitro viability.Therefore, this study was aimed to evaluate the effect of Zebularine on the donor nucleus and cloned embryos of yak.Results showed that there was no affect of cellular viability and proliferation after agent treatment, and the treatment of fibroblasts with Zebularine increased histone acetylation of H3K9ac, but decreased the level of DNA methylation of Oct-4 and Sox-2 promoter regions.The developmental competence and cryosurvival embryos were significantly improved after agent treatment.The relative expression of Oct-4 and Sox-2 were increased significantly, along with significantly decreased the expression level of Dnmt-1 and Hdac-1 when fibroblasts and cloned embryos treated with Zebularine.Above results indicated that treatment with Zebularine has modified epigenetic status of yak fibroblasts, subsequently enhancing in vitro developmental potential and quality of cloned embryos in yak.
Reprogramming SCNT epigenetic development yak
Xiong Xianrong Lan Daoliang Li Jian Zi Xiangdong
College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan, 61 Research Institute of Qinghai-Tibetan Plateau, Southwest University for Nationalities, Chengdu, Sich College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan, 61
国际会议
The 5th International Conference on Yak(第五届国际耗牛大会)
兰州
英文
184-191
2014-08-27（万方平台首次上网日期，不代表论文的发表时间）