Identification of Cyanobacterial Communities and Their Microcystin Congeners in Soda Pits at Nyala Magnesite Mine,South Africa
Cyanobacterial species are known to inhabit adverse environments ranging such as brackish to freshwater. Here we report on the identity of cyanobacterial species and their microcystin congeners that were found in soda pits of an abandoned Nyala Magnesite mine, Limpopo province, South Africa. The samples were collected from soda pits 1 and 3 (water) and pit 3 (sediments) which was later cultured in BG 11 liquid media, incubated at room temperature under continuous light. The fluid samples were introduced to FlowCAM instrument to obtain images for subsequent identification. The algal species that were found in the soda pits were∶ Microcystis, Oscillatoria, Anabaena, Cylindrospermopsis and Phormidium. The microcystin congeners were identified by High Performance Liquid Chromatograph-Diode Array Detector (HPLC-DAD). The HPLC-DAD analysis showed the presence microcystin congeners LR, YR, LY and their range levels of 24. 35 to 102. 71, 18. 16 and 28. 93 ppb respectfully. The microcystin RR was absent. The microcystin LR level exceeds the WHO guidelines of 1 ppb. The availability water in the soda pits has attracted the local communities to use the soda pits for fishing, stock watering and recreational swimming. Thus the presence of these microcystins congeners has the potential to cause harm to the local communities.
cyanobacterial communities microcystin congeners abandoned alkaline mine tailings Microcystis
Mulalo Grace Khabubu Jabulani Ray Gumbo
Department of Hydrology and Water Resources;University of Venda, P/Bag x5050, Thohoyandou, 0950, South Africa
国际会议
The 12th Congress of International Mine Water Association,IMWA2014(第十二届国际矿井水大会)
徐州
英文
384-388
2014-08-18（万方平台首次上网日期，不代表论文的发表时间）