A Photostable AIE Luminogen for Multifunctional Three-photon Bioimaging
In this study we realized multiphoton bioimaging,by utilizing a kind of fluorescent agent with aggregation induced emission(AIE)feature.The luminogen is called tetraphenylethene-triphenylphosphonium(TPETPP),and it is a highly specific mitochondria tracker.We studied its photostability under three-photon excitation,and found that even under long-term excitation,the three-photon fluorescence signals of TPETPP in the cells still kept very bright.Furthermore,under the same 1020 nm femtosecond excitation,TPETPP assisted three-photon imaging has higher spatial resolution than two-photon imaging of MitoTracker red FM(MT),which is a commercially available mitochondria imaging agent.
Zhenfeng Zhu Chris Wai Tung Leung Xinyuan Zhao
Centre for Optical and Electromagnetic Research Zhejiang Provincial Key Laboratory for Sensing Techn Department of Chemistry,The Hong Kong University of Science and Technology Clear Water Bay,Kowloon,H Bioelectromagnetics Laboratory,School of Medicine Zhejiang University,Zijingang Campus,Hangzhou 3100
国际会议
Progress in Electromagnetics Research Symposium 2014(2014年电磁学研究新进展学术研讨会)
广州
英文
2417-2420
2014-08-01（万方平台首次上网日期，不代表论文的发表时间）