Chloroplast Simple Sequence Repeat (cp-SSR) Analysis for Determining the Origin of J.hopeiensis
To define the source of Ma walnut (J.hopeiensis), 10 chloroplast simple sequence repeat (cp-SSR) primers were used to amplify SSR fragments from 7 samples of J.regia, 8 samples of J.mandshurica, 34 samples of J.hopeiensis, and 1 hybid of J.mandshurica♀×J.regia(♂).The results showed that four cpSSR loci were polymorphic.According to alleles of all samples generated by the four polymorphic cpSSR loci, the genotypes of the hybrid of J.mandshurica♀×J.regia(♂) and 24 samples of J.hopeiensis were the same to that of J.mandshurica.Only 1 sample of J.hopeiensis named Yanqingmahetao-2 had the same genotype as J.regia.The genotypes of 9 samples of J.hopeiensis from Shaanxi were the same as that of J.mandshurica in 3 polymorphic cp-SSR loci, while they were the same as that of J.regia in 1 polymorphic cp-SSR locus.These results indicated that J.hopeiensis originated mainly from the natural hybrid of J.mandshurica♀×J.regia(♂).while 97.1% of J.hopeiensis came from the hybrid of J.mandshurica♀×J.regia(♂), while 3% of J.hopeiensis came from J.regia♀ ×J.mandshurica(♂) in the wild population of J.hopeiensis.Furthermore, genetic difference in cp-SSR existed between J.hopeiensis from Shaanxi and those from Beijing and Hebei.
J.hopeiensis J.regia J.mandshurica origin cp-SSR
J.X.Qi C.L.Wu N.G.Dong Y.H.Chen Y.B.Hao
Institute of Forestry and Pomology Beijing Academy of Agriculture and Forestry Science Beijing 100093 China
国际会议
The 7th International Walnut Symposium(第七届世界核桃大会)
山西汾阳
英文
35-40
2013-07-20（万方平台首次上网日期，不代表论文的发表时间）