Developing a Photomixotrophic System for Micropropagation of Persian Walnut
Plantlets grown in conventional tissue culture systems usually encounter physiological and anatomical abnormalities including inability of photosynthesis, low chlorophyll content, open stomata, lack of cuticle layer in the leaf, abnormal xylem parenchyma, etc.Photoautotrophic and photomixotrophic systems could diminish these problems.Photomixotrophy is the nutritional type where living organisms use not only endogenous but also exogenous carbohydrates as an energy source.The purpose of this study was to increase the chlorophyll content and photosynthetic ability of in vitro grown shoots and better adaptation of walnut plantlets.Natural ventilation, sucrose, chelated iron and supporting materials were tested.Walnut apical leaf buds were cultured in without ventilation and natural ventilation vessels (vitrovent vessels).Natural ventilation had a significant effect on most of the growth indices.Also, different levels of sucrose and supporting materials had significant effect on growth characteristics.Plants grown in ventilation vessels containing vermiculite and 15 g/L sucrose were lusher.Plantlets grown in medium containing Fe-EDDHA had more growth than plantlets grown in the Fe-Na2EDTA medium.Natural ventilation significantly increased content of chlorophyll.By using of 15 g/L sucrose, the chlorophyll b and total chlorophyll significantly increased.Also, vermiculite increased the content of chlorophyll a, b and total significantly.In general, using ventilated vessels containing vermiculite and 15 g/L sucrose significantly increased the chlorophyll a, b and total chlorophyll.Stomata under non-ventilated condition were spherical with wide openings whereas, those in ventilated vessels were elliptical with narrow openings.Natural leaf stomata were observed in ventilated vessels containing vermiculite.As a result, ventilated vessels with 30 g/L of sucrose and vermiculite and 79 mg/L of Fe-EDDH produced healthy plantlets.
tissue culture ventilation vermiculite chlorophyll iron
K.Vahdati A.Hassankhah
Department of Horticulture University of Tehran Aburaihan Campus,Pakdasht Iran
国际会议
The 7th International Walnut Symposium(第七届世界核桃大会)
山西汾阳
英文
181-187
2013-07-20（万方平台首次上网日期，不代表论文的发表时间）